Why BG-11 medium is used for cyanobacteria?

Why BG-11 medium is used for cyanobacteria?

For heterocyst-forming cyanobacteria like Anabaena, the main Nitrogen source (NaNO3) is completely removed from the BG11 medium formulation to preserve the N2- fixation ability. Thus, BG11(N-) is commonly used for culturing diazotrophic cyanobacteria.

What is BG-11 medium?

BG11 Broth is a universal medium for the cultivation and maintenance of blue green algae (cyanobacteria).

How do you make BG11 agar?

Combine the following solutions and adjust pH to 7,5 (Use 1.0M HCl or NaOH). Stock Per liter of medium Stock 1 10 ml Stock 2 10 ml Stock 3 10 ml Na2CO3 0,02 g Stock 4 1,0 ml Page 2 NaNO3 1.5 g (For 100x BG-11 solution don’t add NaNO3) For solid media: add 1.5% agar to the liquid BG11 media.

How do you make bold basal medium?

The standard medium is prepared as follows: Add 1.0 mL of 0.1% Sulfuric Acid solution to 1 liter of DI water along with 0.705 g of B1675. The final solution pH is adjusted to 6.6 +/- 0.1 with KOH (Stein, 1973).

What is the use of ferric ammonium citrate?

Ammonium ferric citrate has a range of uses, including: As a food ingredient, it has an INS number, and is used as an acidity regulator. Most notably used in the Scottish beverage Irn-Bru. Water purification.

How do you test for cyanobacteria?

A simple test you can do is the “stick test.” Find a sturdy stick; long enough to thrust into the surface mat without letting you fall in, and see what comes back out on it. If the stick comes out looking like you thrust it in a can of paint, the mat on your lake is likely to be a blue-green algae scum.

How do cyanobacteria photosynthesize?

Cyanobacteria use the energy of sunlight to drive photosynthesis, a process where the energy of light is used to split water molecules into oxygen, protons, and electrons. Cyanobacteria get their color from the bluish pigment phycocyanin, which they use to capture light for photosynthesis.

What is a basal medium?

An unsupplemented medium which promotes the growth of many types of microorganisms which do not require any special nutrient supplements. Form. Liquid (202)

What is bold basal medium?

Bold’s Basal Medium (BBM) is a freshwater algae medium that has been used to grow a variety of green algal cultures (e.g. Trichosarcina, Chlorococcum, and Chlorella) without the need for soil-extract or vitamins (Brown et al., 1964; Nichols and Bold, 1965).

Is ferric ammonium citrate soluble?

Ammonium ferric citrate has the formula (NH4)5[Fe(C6H4O7)2]. A distinguishing feature of this compound is that it is very soluble in water, in contrast to ferric citrate which is not very soluble. In its crystal structure each moiety of citric acid has lost four protons.

What is the use of ferric ammonium citrate folic acid and vitamin b12 syrup?

Adfe Syrup is a Syrup manufactured by ADROIT HEALTH. It is commonly used for the diagnosis or treatment of anemia due to folic acid deficiency, folate deficiency, increased requirement of folate in the body during pregnancy .