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How does the Invitrogen Quant iT assay kit work?

How does the Invitrogen Quant iT assay kit work?

Invitrogen Quant-iT assay kits utilize advanced fluorophores that become fluorescent upon binding to DNA, RNA, or proteins; the fluorescence intensity of the resulting complex depends directly on the amount of the target molecule in the sample.

Is there a Quant-iT dsDNA assay for research?

A Quant-iT™ dsDNA Assay Kit with a range of 2-1000 ng of dsDNA is available (Q33130), and we also offer a Quant-iT™ RNA Assay Kit (Q33140). For Research Use Only. Not for use in diagnostic procedures. Store in refrigerator (2–8°C) and protect from light.

Which is the most accurate DNA quantitation kit?

The Quant-iT™ High-Sensitivity dsDNA Assay Kit makes DNA quantitation easy and accurate. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. The assay is highly selective for double-stranded DNA over RNA, and in the range of 0.2-100 ng of DNA, the fluorescence signal is linear.

How does Qubit DNA and RNA kit work?

The Qubit DNA and RNA kits only quantify the amount of either DNA or RNA in the sample. The Qubit fluorometer cannot take absorbance readings to provide a A260/A280 ratio or detect protein in nucleic acid samples. This can be done with the NanoDrop instrument.

How does the Quant-iT ssDNA assay kit work?

Our Quant-iT™ OliGreen® ssDNA Assay Kit contains an ultrasensitive, green-fluorescent nucleic acid stain for quantitating oligonucleotides and single-stranded DNA (ssDNA) in solution. In addition to the dye, the kit also includes buffer and an oligonucleotide standard.

Can a Quant iT kit be used with a Qubit fluorometer?

In addition, the new Quant-iT kits (labeled as “for use with the Qubit Fluorometer”) have standards formulated to be compatible with the Qubit fluorometer internal algorithms for the respective assays. The Qubit Fluorometer-compatible kits are also less expensive per assay if you are processing fewer than 20 samples at a time.