Questions and answers

Which animal produces green fluorescent protein?

Which animal produces green fluorescent protein?

Green fluorescent protein (GFP) is a protein in the jellyfish Aequorea Victoria that exhibits green fluorescence when exposed to light.

What is a GFP mouse?

Green fluorescent protein (GFP) found in Aequorea victoria absorbs blue light and emits green fluorescence without exogenous substrates or co-factors. Transgenic mice were produced using the GFP coding sequence, ligated with the chicken beta-actin promoter.

What are overexpressing transgenic mice?

Generate a mouse model in which an exogenous gene is introduced and overexpressed. An overexpression model can be used to investigate gene functions, promoter functions, or model the pathogenesis of human disease.

Why does the glow gene from jellyfish work in transgenic mice?

These mice are glowing because scientists inserted a gene found in certain bioluminescent jellyfish into their DNA. That gene is a recipe for a protein that glows green when hit by blue or ultraviolet light. As a result, their skin, eyes and organs give off an eerie light.

What does green fluorescent protein do in nature?

But GFP’s natural function has remained a mystery — as well as jellyfish, it is naturally present in a wide range of animals and plants, including some species of coral and fish. Now it seems that the green protein can donate electrons in a process powered by light, to molecules that like to accept electrons.

Why are fluorescent proteins important?

The function of the fluorescent protein is to act as a bioluminescence resonance energy transfer (BRET) acceptor that converts the otherwise blue emission of the bioluminescent protein into a longer wavelength green emission.

How do mammalian cells overexpress proteins?

Popular Answers (1)

  1. clone your gene of interest into an appropriate vector under control of the appropriate promoter.
  2. transfect or transduce your cells of interest using the appropriate protocol.
  3. begin antibiotic selection to enrich for stably integrated clones.

What are transgenic lines?

The generation of a zebrafish transgenic line allows the expression of exogenous coding sequences (CDS) (e.g. transcription factors, CDS of human genes, fluorescent reporters, sensors to test environmental changes, optogenetic tools) under the control of tissue-specific promoters.