What is the role of B phenylethyl alcohol in pea?

In your own words, what is the role of b-phenylethyl alcohol in PEA and how does it work? Phenylethyl alcohol is the selective agent in PEA. It inhibits the growth of Gram-negative organisms by disrupting the cell membrane, which affects osmotic and pH homeostasis.

What can phenylethyl alcohol do to the cytoplasmic membrane of bacteria?

Principle. Phenylethyl alcohol agar (PEA) is a selective medium that permits the growth of gram-positive cocci while inhibiting most gram-negative organisms. PEA alters the membrane permeability, of Gram-negative bacteria allowing influx of otherwise blocked molecules.

Is chocolate agar selective or differential?

Chocolate Agar is an enriched general- purpose medium that supports the growth of most fastidious and non-fastidious organisms. Because it is a non-selective medium, resident flora from clinical specimens may overgrow potential fastidious pathogens, such as Neisseria species.

What ingredients are in BEA supplies?

The ingredients of Bile Esculin agar (BEA) contains, Beef Extract, Pancreatic Digest of Gelatin, Oxgall, Ferric Citrate, Esculin, Agar, and Distilled water.

What grows on Lkv?

If used properly, LKV agar will support good growth of some obligately anaerobic gram-negative bacteria (e.g. Prevotella melaningenica and Bacteroides fragilis) and inhibit the growth of facultatively anaerobic bacteria (e.g. Escherichia coli, Proteus mirabilis, and Staphylococcus aureus) and gram-positive obligate …

How does phenylethyl alcohol agar work?

Phenylethyl alcohol agar (PEA) is a selective medium used to cultivate Gram positive organisms. The active ingredient, phenylethyl alcohol, inhibits or markedly reduces growth of Gram negative organisms by interfering with DNA synthesis.

What type of bacteria does PA agar inhibit?

PEA agar is a selective medium that is used for the isolation of gram- positive Staphylococcus species andStreptococcus species from clinical specimens or specimens that contain mixtures of bacterial flora (2). Typically PEA agar is used to inhibit the common contaminants such as Escherichia coli and Proteus species.

What is CNA Agar made of?

The selective/ inhibitory agent of CNA is the antibiotic naladixic acid, a quinolone drug similar to Cipro or Levaquin. This medium is basically blood agar, containing 5% sheep’s blood mixed with either TSA base or Columbia agar base.

How much alcohol is in Phenylethyl Alcohol Agar?

Difco™ & BBL™ Manual, 2nd Edition Phenylethyl Alcohol Agar Phenylethyl Alcohol Agar with 5% Sheep Blood Intended Use

How is phenylethyl alcohol used as a selective medium?

Phenylethyl Alcohol Agar Phenylethyl alcohol agar (PEA) is a selective medium used to cultivate Gram positive organisms. The active ingredient, phenylethyl alcohol, inhibits or markedly reduces growth of Gram negative organisms by interfering with DNA synthesis. PEA also prevents Proteus species from swarming across the surface of the agar.

How is phenylethyl alcohol used to cultivate Gram positive organisms?

Phenylethyl alcohol agar (PEA) is a selective medium used to cultivate Gram positive organisms. The active ingredient, phenylethyl alcohol, inhibits or markedly reduces growth of Gram negative organisms by interfering with DNA synthesis. PEA also prevents Proteus species from swarming across the surface of the agar.

What does 4.25% phenylethyl alcohol look like?

Dehydrated Appearance: Slightly moist and softly clumped, resembling “brown sugar” in consistency and appearance. Solution: 4.25% solution, soluble in purified water upon boiling. Solution is light to medium, yellow to tan, clear to slightly hazy.