What is cell culture basics?

Cell culture refers to the removal of cells from an animal or plant and their subsequent growth in a favorable artificial environment. At this stage, the cells have to be subcultured (i.e., passaged) by transferring them to a new vessel with fresh growth medium to provide more room for continued growth.

What does passaging cells mean?

Subculturing, also referred to as passaging cells, is the removal of the medium and transfer of cells from a previous culture into fresh growth medium, a procedure that enables the further propagation of the cell line or cell strain.

What is the use of trypsin in cell culture?

Trypsinization is the process of cell dissociation using trypsin, a proteolytic enzyme which breaks down proteins, to dissociate adherent cells from the vessel in which they are being cultured. When added to a cell culture, trypsin breaks down the proteins which enable the cells to adhere to the vessel.

What are the basic precautions needed for cell culture work?

This goes without saying, you must provide a barrier between your cells and the non-sterile environment that is your laboratory (or indeed yourself). Using gloves, lab-coats and hoods does just that. Use your lab-coats only inside your cell culture lab and have them cleaned often.

What are cellular fractions?

Cell fractionation is the process used to separate cellular components while preserving individual functions of each component. This is a method that was originally used to demonstrate the cellular location of various biochemical processes.

How do you prepare media for cell culture?

The following steps outline the proper preparation of media for tissue culture:

1. Mix a powdered medium with the appropriate amount of water.
2. If you are mixing for a 1-liter medium, then fill a beaker with 800ml distilled water.
3. Add 30g sucrose.
4. Set the PH at 5.8.
5. Add agar to the beaker (8g).
6. Add hormone (if using).

Why is PBS used in cell culture?

Phosphate buffered saline (PBS) is a non-toxic solution used in many biological laboratories. Unlike water, PBS prevents cells rupturing or shrivelling up due to osmosis.

How do you take care of cell culture?

Avoid keeping cell lines continually in culture without returning to frozen stock. Avoid cell cultures from becoming fully confluent. Always sub-culture at 70-80% confluency or as advised on the ECACC cell culture data sheet. Do not allow media to go out of date.

What do you need to know about GIBCO cell culture?

Gibco Cell Culture Basics is an introduction to cell culture, covering topics such as laboratory setup, safety, and aseptic technique. You’ll also find basic cell culture techniques and methods for passaging, freezing, and thawing cultured cells.

What do you need to know about cell culture?

Culture Conditions Culture conditions vary widely for each cell type, but the artificial environment in which the cells are cultured invariably consists of a suitable vessel containing a substrate or medium that supplies the essential nutrients (amino acids, carbohydrates, vitamins, minerals), growth factors, hormones, and gases (O 2, CO

What are the dangers of working in cell culture?

In addition to the safety risks common to most everyday work places such as electrical and fire hazards, a cell culture laboratory has a number of specific hazards associated with handling and manipulating human or animal cells and tissues, as well as toxic, corrosive, or mutagenic solvents and reagents.

Where can I find the cell culture basics companion Handbook?

Cell Culture Basics Companion Handbook is a supplement to the Cell Culture Basics instructional videos available online at www.lifetechnologies.com/cellculturebasics. The handbook and videos are intended as an introduction to cell culture basics.