What are the common methods of drug administration in mice?

Among several possibilities for the administration of substances to mice, the most common routes are subcutaneous, intraperitoneal or intravenous injection. The intramuscular administration is not recommended, as the muscle of the mouse is too small.

Which route is used for administration of Phenobarbitone subcutaneous intramuscular intravenous intraperitoneal?

Phenobarbital sodium may be administered intramuscularly or intravenously as an anticonvulsant for emergency use. When administered intravenously, it may require 15 or more minutes before reaching peak concentrations in the brain.

How much fluid can a mouse be given subcutaneously over a 24 hour period?

This requirement is estimated as 100 ml/kg in a 1 kg or smaller animal. Body weight (kg) x 100 ml = Fluid volume (ml) per 24 hour 8. Twenty-four hour fluid volume replacements should be divided into 2 or 3 treatments over the 24 hour period.

How do you ip injection a mouse?

For mice: Tilt the mouse with its head slightly toward the ground so that its head is lower than its hind end. This allows the abdominal viscera to shift cranially and minimize accidental puncture of abdominal organs at site of injection.

How much saline can you give a mouse?

Add the saline slowly at first (a few drops at a time) with stirring to avoid the drug crashing out of solution. Not too vigorously or the solution will foam. You can add the saline more rapidly once the volume increases. You will now have a 1 mg/ml solution which you can dose IP at 10 ml/kg or 200 uL for a 20 g mouse.

Why is pentobarbital preferred over Phenobarbitone?

Pentobarbital is often subject to comparison with phenobarbital, another barbiturate, in the use of refractory status epilepticus. Studies have found that pentobarbital is superior in that it has faster brain penetration and a shorter half-life, making it the treatment of choice.

Which site should sc injection be given?

Subcutaneous tissue is all over your body, but the most common areas for subcutaneous injections are:

1. the upper outer area of the arm.
2. the front and outer sides of the thighs.
3. the abdomen, except for a 2 inch area around the navel.
4. the upper outer area of the buttocks.
5. the upper hip.

What is the maximum volume for subcutaneous injection?

Administration into the subcutaneous (SC) tissue is a typical route of delivery for therapeutic proteins, especially for frequent treatments, long-term regimens, or self-administration. It is currently believed that the maximum volume for SC injections is approximately 1.5 mL.

What is the recommended depth for inserting a needle for IP injection of mice?

~5mm
Insert needle, direct tip toward head, bevel up, at 15-30 degree angle, to depth of ~5mm.

Where do you inject mice?

Mice & Rats -Intramuscular Injection (IM) IM injections are administered in the thigh muscles of the hind limb. Caution must be used to avoid injury to the sciatic nerve, which is located in close proximity to the femur. 1. Restrain the animal by grasping the skin along its back with your left hand (if right-handed).

How do I give myself a subcutaneous injection?

Hold the syringe in your dominant (stronger) hand, as if holding a pencil, one inch above the injection site at a 90 degree angle to the skin. Quickly dart (stab) the needle through the skin into the fat tissue. 4. Use your non-dominant (weaker) hand to slowly push down on the plunger to inject the medication.